Review





Similar Products

pmrx ip gfp lc3 rfp  (Addgene inc)
93
Addgene inc pmrx ip gfp lc3 rfp
Pmrx Ip Gfp Lc3 Rfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmrx ip gfp lc3 rfp/product/Addgene inc
Average 93 stars, based on 1 article reviews
pmrx ip gfp lc3 rfp - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
fluorescent probe gfp lc3 rfp lc3δg  (Addgene inc)
93
Addgene inc fluorescent probe gfp lc3 rfp lc3δg
A, Schematic diagram of autophagy pathway and the mechanism of action of bafilomycin A1 to inhibit autophagy. B, C, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM (C) and REH (D) <t>cells</t> <t>expressing</t> <t>GFP-LC3-RFP-LC3ΔG</t> autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h with or without 50 nM of bafilomycin A1 treatment (n=7 independent experiments). D, Immunoblot analysis showing changes in autophagy markers in human CD19 + (huCD19 + ) SEM cells isolated from the bone marrow (BM) and the brain (CNS) (n=2 biological replicates). E, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) with or without 1 μM of MRT403 treatment (n=3 independent experiments). F, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) or hypoxia (1% O 2 ) with or without 50 nM of bafilomycin A1 treatment (n=3 independent experiments). G, Immunoblot analysis showing deletion of ULK1 and changes in autophagy markers in SEM cells cultured in RPMI. H, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM vector control (CTRL) and ULK1 knockout (KO) single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). I, Immunoblot analysis showing deletion of ATG7 and changes in autophagy markers in SEM cells cultured in RPMI. J, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM CTRL and ATG7 KO single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Tukey’s multiple comparison test (B, C, E, F, H, J).
Fluorescent Probe Gfp Lc3 Rfp Lc3δg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescent probe gfp lc3 rfp lc3δg/product/Addgene inc
Average 93 stars, based on 1 article reviews
fluorescent probe gfp lc3 rfp lc3δg - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
noboru mizushima  (Addgene inc)
93
Addgene inc noboru mizushima
A, Schematic diagram of autophagy pathway and the mechanism of action of bafilomycin A1 to inhibit autophagy. B, C, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM (C) and REH (D) <t>cells</t> <t>expressing</t> <t>GFP-LC3-RFP-LC3ΔG</t> autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h with or without 50 nM of bafilomycin A1 treatment (n=7 independent experiments). D, Immunoblot analysis showing changes in autophagy markers in human CD19 + (huCD19 + ) SEM cells isolated from the bone marrow (BM) and the brain (CNS) (n=2 biological replicates). E, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) with or without 1 μM of MRT403 treatment (n=3 independent experiments). F, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) or hypoxia (1% O 2 ) with or without 50 nM of bafilomycin A1 treatment (n=3 independent experiments). G, Immunoblot analysis showing deletion of ULK1 and changes in autophagy markers in SEM cells cultured in RPMI. H, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM vector control (CTRL) and ULK1 knockout (KO) single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). I, Immunoblot analysis showing deletion of ATG7 and changes in autophagy markers in SEM cells cultured in RPMI. J, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM CTRL and ATG7 KO single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Tukey’s multiple comparison test (B, C, E, F, H, J).
Noboru Mizushima, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/noboru mizushima/product/Addgene inc
Average 93 stars, based on 1 article reviews
noboru mizushima - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
pmrx ipgfp lc3 rfp lc3δg plasmid  (Addgene inc)
93
Addgene inc pmrx ipgfp lc3 rfp lc3δg plasmid
A, Schematic diagram of autophagy pathway and the mechanism of action of bafilomycin A1 to inhibit autophagy. B, C, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM (C) and REH (D) <t>cells</t> <t>expressing</t> <t>GFP-LC3-RFP-LC3ΔG</t> autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h with or without 50 nM of bafilomycin A1 treatment (n=7 independent experiments). D, Immunoblot analysis showing changes in autophagy markers in human CD19 + (huCD19 + ) SEM cells isolated from the bone marrow (BM) and the brain (CNS) (n=2 biological replicates). E, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) with or without 1 μM of MRT403 treatment (n=3 independent experiments). F, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) or hypoxia (1% O 2 ) with or without 50 nM of bafilomycin A1 treatment (n=3 independent experiments). G, Immunoblot analysis showing deletion of ULK1 and changes in autophagy markers in SEM cells cultured in RPMI. H, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM vector control (CTRL) and ULK1 knockout (KO) single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). I, Immunoblot analysis showing deletion of ATG7 and changes in autophagy markers in SEM cells cultured in RPMI. J, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM CTRL and ATG7 KO single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Tukey’s multiple comparison test (B, C, E, F, H, J).
Pmrx Ipgfp Lc3 Rfp Lc3δg Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmrx ipgfp lc3 rfp lc3δg plasmid/product/Addgene inc
Average 93 stars, based on 1 article reviews
pmrx ipgfp lc3 rfp lc3δg plasmid - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
n a recombinant dna pmrx ip gfp lc3 rfp lc3δg addgene  (Addgene inc)
95
Addgene inc n a recombinant dna pmrx ip gfp lc3 rfp lc3δg addgene
A, Schematic diagram of autophagy pathway and the mechanism of action of bafilomycin A1 to inhibit autophagy. B, C, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM (C) and REH (D) <t>cells</t> <t>expressing</t> <t>GFP-LC3-RFP-LC3ΔG</t> autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h with or without 50 nM of bafilomycin A1 treatment (n=7 independent experiments). D, Immunoblot analysis showing changes in autophagy markers in human CD19 + (huCD19 + ) SEM cells isolated from the bone marrow (BM) and the brain (CNS) (n=2 biological replicates). E, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) with or without 1 μM of MRT403 treatment (n=3 independent experiments). F, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) or hypoxia (1% O 2 ) with or without 50 nM of bafilomycin A1 treatment (n=3 independent experiments). G, Immunoblot analysis showing deletion of ULK1 and changes in autophagy markers in SEM cells cultured in RPMI. H, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM vector control (CTRL) and ULK1 knockout (KO) single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). I, Immunoblot analysis showing deletion of ATG7 and changes in autophagy markers in SEM cells cultured in RPMI. J, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM CTRL and ATG7 KO single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Tukey’s multiple comparison test (B, C, E, F, H, J).
N A Recombinant Dna Pmrx Ip Gfp Lc3 Rfp Lc3δg Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/n a recombinant dna pmrx ip gfp lc3 rfp lc3δg addgene/product/Addgene inc
Average 95 stars, based on 1 article reviews
n a recombinant dna pmrx ip gfp lc3 rfp lc3δg addgene - by Bioz Stars, 2026-05
95/100 stars
  Buy from Supplier
pmrx ip 68 gfp lc3 rfp  (Addgene inc)
93
Addgene inc pmrx ip 68 gfp lc3 rfp
A, Schematic diagram of autophagy pathway and the mechanism of action of bafilomycin A1 to inhibit autophagy. B, C, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM (C) and REH (D) <t>cells</t> <t>expressing</t> <t>GFP-LC3-RFP-LC3ΔG</t> autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h with or without 50 nM of bafilomycin A1 treatment (n=7 independent experiments). D, Immunoblot analysis showing changes in autophagy markers in human CD19 + (huCD19 + ) SEM cells isolated from the bone marrow (BM) and the brain (CNS) (n=2 biological replicates). E, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) with or without 1 μM of MRT403 treatment (n=3 independent experiments). F, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) or hypoxia (1% O 2 ) with or without 50 nM of bafilomycin A1 treatment (n=3 independent experiments). G, Immunoblot analysis showing deletion of ULK1 and changes in autophagy markers in SEM cells cultured in RPMI. H, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM vector control (CTRL) and ULK1 knockout (KO) single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). I, Immunoblot analysis showing deletion of ATG7 and changes in autophagy markers in SEM cells cultured in RPMI. J, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM CTRL and ATG7 KO single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Tukey’s multiple comparison test (B, C, E, F, H, J).
Pmrx Ip 68 Gfp Lc3 Rfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmrx ip 68 gfp lc3 rfp/product/Addgene inc
Average 93 stars, based on 1 article reviews
pmrx ip 68 gfp lc3 rfp - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
pmrx ip gfp lc3 rfp construct  (Addgene inc)
93
Addgene inc pmrx ip gfp lc3 rfp construct
A, Schematic diagram of autophagy pathway and the mechanism of action of bafilomycin A1 to inhibit autophagy. B, C, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM (C) and REH (D) <t>cells</t> <t>expressing</t> <t>GFP-LC3-RFP-LC3ΔG</t> autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h with or without 50 nM of bafilomycin A1 treatment (n=7 independent experiments). D, Immunoblot analysis showing changes in autophagy markers in human CD19 + (huCD19 + ) SEM cells isolated from the bone marrow (BM) and the brain (CNS) (n=2 biological replicates). E, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) with or without 1 μM of MRT403 treatment (n=3 independent experiments). F, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) or hypoxia (1% O 2 ) with or without 50 nM of bafilomycin A1 treatment (n=3 independent experiments). G, Immunoblot analysis showing deletion of ULK1 and changes in autophagy markers in SEM cells cultured in RPMI. H, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM vector control (CTRL) and ULK1 knockout (KO) single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). I, Immunoblot analysis showing deletion of ATG7 and changes in autophagy markers in SEM cells cultured in RPMI. J, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM CTRL and ATG7 KO single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Tukey’s multiple comparison test (B, C, E, F, H, J).
Pmrx Ip Gfp Lc3 Rfp Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmrx ip gfp lc3 rfp construct/product/Addgene inc
Average 93 stars, based on 1 article reviews
pmrx ip gfp lc3 rfp construct - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
autophagy flux assay 161  (Addgene inc)
93
Addgene inc autophagy flux assay 161
A, Schematic diagram of autophagy pathway and the mechanism of action of bafilomycin A1 to inhibit autophagy. B, C, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM (C) and REH (D) <t>cells</t> <t>expressing</t> <t>GFP-LC3-RFP-LC3ΔG</t> autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h with or without 50 nM of bafilomycin A1 treatment (n=7 independent experiments). D, Immunoblot analysis showing changes in autophagy markers in human CD19 + (huCD19 + ) SEM cells isolated from the bone marrow (BM) and the brain (CNS) (n=2 biological replicates). E, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) with or without 1 μM of MRT403 treatment (n=3 independent experiments). F, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) or hypoxia (1% O 2 ) with or without 50 nM of bafilomycin A1 treatment (n=3 independent experiments). G, Immunoblot analysis showing deletion of ULK1 and changes in autophagy markers in SEM cells cultured in RPMI. H, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM vector control (CTRL) and ULK1 knockout (KO) single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). I, Immunoblot analysis showing deletion of ATG7 and changes in autophagy markers in SEM cells cultured in RPMI. J, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM CTRL and ATG7 KO single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Tukey’s multiple comparison test (B, C, E, F, H, J).
Autophagy Flux Assay 161, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/autophagy flux assay 161/product/Addgene inc
Average 93 stars, based on 1 article reviews
autophagy flux assay 161 - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
gfp lc3 rfp probe  (Addgene inc)
93
Addgene inc gfp lc3 rfp probe
A, Schematic diagram of autophagy pathway and the mechanism of action of bafilomycin A1 to inhibit autophagy. B, C, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM (C) and REH (D) <t>cells</t> <t>expressing</t> <t>GFP-LC3-RFP-LC3ΔG</t> autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h with or without 50 nM of bafilomycin A1 treatment (n=7 independent experiments). D, Immunoblot analysis showing changes in autophagy markers in human CD19 + (huCD19 + ) SEM cells isolated from the bone marrow (BM) and the brain (CNS) (n=2 biological replicates). E, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) with or without 1 μM of MRT403 treatment (n=3 independent experiments). F, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) or hypoxia (1% O 2 ) with or without 50 nM of bafilomycin A1 treatment (n=3 independent experiments). G, Immunoblot analysis showing deletion of ULK1 and changes in autophagy markers in SEM cells cultured in RPMI. H, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM vector control (CTRL) and ULK1 knockout (KO) single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). I, Immunoblot analysis showing deletion of ATG7 and changes in autophagy markers in SEM cells cultured in RPMI. J, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM CTRL and ATG7 KO single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Tukey’s multiple comparison test (B, C, E, F, H, J).
Gfp Lc3 Rfp Probe, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gfp lc3 rfp probe/product/Addgene inc
Average 93 stars, based on 1 article reviews
gfp lc3 rfp probe - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
pmrxip gfp lc3 rfp lc3δg  (Addgene inc)
95
Addgene inc pmrxip gfp lc3 rfp lc3δg
A, Schematic diagram of autophagy pathway and the mechanism of action of bafilomycin A1 to inhibit autophagy. B, C, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM (C) and REH (D) <t>cells</t> <t>expressing</t> <t>GFP-LC3-RFP-LC3ΔG</t> autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h with or without 50 nM of bafilomycin A1 treatment (n=7 independent experiments). D, Immunoblot analysis showing changes in autophagy markers in human CD19 + (huCD19 + ) SEM cells isolated from the bone marrow (BM) and the brain (CNS) (n=2 biological replicates). E, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) with or without 1 μM of MRT403 treatment (n=3 independent experiments). F, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) or hypoxia (1% O 2 ) with or without 50 nM of bafilomycin A1 treatment (n=3 independent experiments). G, Immunoblot analysis showing deletion of ULK1 and changes in autophagy markers in SEM cells cultured in RPMI. H, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM vector control (CTRL) and ULK1 knockout (KO) single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). I, Immunoblot analysis showing deletion of ATG7 and changes in autophagy markers in SEM cells cultured in RPMI. J, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM CTRL and ATG7 KO single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Tukey’s multiple comparison test (B, C, E, F, H, J).
Pmrxip Gfp Lc3 Rfp Lc3δg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmrxip gfp lc3 rfp lc3δg/product/Addgene inc
Average 95 stars, based on 1 article reviews
pmrxip gfp lc3 rfp lc3δg - by Bioz Stars, 2026-05
95/100 stars
  Buy from Supplier

Image Search Results


A, Schematic diagram of autophagy pathway and the mechanism of action of bafilomycin A1 to inhibit autophagy. B, C, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM (C) and REH (D) cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h with or without 50 nM of bafilomycin A1 treatment (n=7 independent experiments). D, Immunoblot analysis showing changes in autophagy markers in human CD19 + (huCD19 + ) SEM cells isolated from the bone marrow (BM) and the brain (CNS) (n=2 biological replicates). E, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) with or without 1 μM of MRT403 treatment (n=3 independent experiments). F, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) or hypoxia (1% O 2 ) with or without 50 nM of bafilomycin A1 treatment (n=3 independent experiments). G, Immunoblot analysis showing deletion of ULK1 and changes in autophagy markers in SEM cells cultured in RPMI. H, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM vector control (CTRL) and ULK1 knockout (KO) single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). I, Immunoblot analysis showing deletion of ATG7 and changes in autophagy markers in SEM cells cultured in RPMI. J, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM CTRL and ATG7 KO single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Tukey’s multiple comparison test (B, C, E, F, H, J).

Journal: bioRxiv

Article Title: Physiological cerebrospinal fluid like medium reveals autophagy dependency of leukaemia in the central nervous system

doi: 10.64898/2026.03.09.709824

Figure Lengend Snippet: A, Schematic diagram of autophagy pathway and the mechanism of action of bafilomycin A1 to inhibit autophagy. B, C, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM (C) and REH (D) cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h with or without 50 nM of bafilomycin A1 treatment (n=7 independent experiments). D, Immunoblot analysis showing changes in autophagy markers in human CD19 + (huCD19 + ) SEM cells isolated from the bone marrow (BM) and the brain (CNS) (n=2 biological replicates). E, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) with or without 1 μM of MRT403 treatment (n=3 independent experiments). F, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) or hypoxia (1% O 2 ) with or without 50 nM of bafilomycin A1 treatment (n=3 independent experiments). G, Immunoblot analysis showing deletion of ULK1 and changes in autophagy markers in SEM cells cultured in RPMI. H, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM vector control (CTRL) and ULK1 knockout (KO) single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). I, Immunoblot analysis showing deletion of ATG7 and changes in autophagy markers in SEM cells cultured in RPMI. J, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM CTRL and ATG7 KO single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Tukey’s multiple comparison test (B, C, E, F, H, J).

Article Snippet: Similarly, to generate stable ALL cell lines expressing the fluorescent probe GFP-LC3-RFP-LC3ΔG we used PMRX-IP-GFP-LC3-RFP which was a gift from Noboru Mizushima (Addgene Plasmid #84573) [ ].

Techniques: Fluorescence, Expressing, Cell Culture, Western Blot, Isolation, Plasmid Preparation, Control, Knock-Out, Comparison